Tracking Genomic Sequences with Unique Identifiers and Markers: A Look at UDIs and UMIs in Next-Generation Sequencing Data Management
In the realm of biotechnology, Twist Bioscience is making waves with its innovative UDI (Unique Dual Index) and UMI (Unique Molecular Identifier) Adapters. These adapters are designed to revolutionise genomic research, empowering scientists to delve deeper into the mysteries of life.
The adapters are meticulously crafted to deliver uniform read counts and minimise barcode collisions, a critical aspect in high-throughput sequencing projects. This feature allows researchers to pool thousands of samples, a boon for studies requiring large sample sizes, such as cancer mutation detection, epigenetic changes analysis, and scaling research without compromising data quality.
Twist's UDI and UMI Adapters are also designed for precision and built for discovery. The company offers a vast array of unique indexes in its High-Throughput (HT) Universal Adapter System, each one empirically tested for uniform read distribution and sequence accuracy. With 3,072 unique indexes available, researchers have a wealth of options to choose from, depending on their specific needs.
The Universal Adapter System, with its 10 bp balanced index designs, reduces misassignment errors in applications like cancer biomarker discovery. Meanwhile, the HT Universal Adapter System, with its 12 bp design, is ideal for automation and large-scale studies like rare disease gene panels.
For ultra-sensitive applications, like detecting rare somatic variants or studying methylation patterns in cell-free DNA, the Twist UMI Adapter System comes into play. This system pairs efficient ligation chemistry with high-fidelity barcoding, ensuring accurate sample demultiplexing even with minor mismatches allowed by bioinformatics pipelines.
In the field of agriculture, Twist's UDI adapters play a crucial role, enabling precise, high-throughput genetic analysis necessary for advanced research in crop genetics and breeding.
Moreover, the Twist Methylation Detection System is compatible with the Twist Methylated UMI Adapters, further expanding the potential applications of these innovative tools. The Methylated UMI Adapters enable accurate deduplication, demonstrating a reduction of false duplication calls by 15% in low-diversity samples.
UMIs, used in next-generation sequencing (NGS) to determine if a sequence came from a unique DNA molecule or is a PCR artifact, are an integral part of Twist's UDI Adapters.
In essence, Twist's UDI and UMI Adapters are key to unlocking huge data sets and making discoveries that change the world. They are reliable, perform reliably, and integrate seamlessly into high-throughput and high-sensitivity applications, making them an invaluable tool in the quest for scientific advancement.
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